상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Characterization of a cell line HFH-T2, producing viral particles, from primary human fetal hepatocytes infected with hepatitis B virus

Authors 

Jae Kyoung ShimDong Wook KimTae Ho ChungJune Ki KimJeong Ill SuhChun ParkYoung Choon LeeTai Wha ChungEun Young SongChul Ho Kim

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2001

Citation 

Journal of Microbiology and Biotechnology, vol. 11, no. 2, pp. 186-192

Keywords 

dan particleenzyme-linked immunosorbent assayfetal hepatocyteHBsAgHBsAg particlehepatitis B virushepatocellular carcinomanatural infectionhepatitis B surface antigenvirus antibody

Abstract 

A primary culture of human fetal hepatocytes was obtained though a therapeutic abortion process at 26 weeks of gestation period. More than 108 cells were seeded on a plastic plate. These hepatocytes were infected with hepatitis B virus (HBV). The HBV was purified from serum of one chronic HBV carrier. Transformed hepatocytes were subcultured in a 10% FBS-supplemented medium. The morphology of the transformed cell was epithelial-like. The cells from the first pass showed signs of early proliferation and had a latent period of more than 3 months after 6-7 passages. After the rest period, the transformed cell proliferated actively and they were subcultured every three days. Transformed hepatocytes were characterized by detection of the HBV transcript by RT-PCR. The secretion of virions from transformed cells was investigated by PCR with the cell medium. Two types of virions secreted into the culture medium were examined by using the transmission electron microscope. Another approach to study the secretion of virions in to culture medium was carried out with HBV antibody. HBsAg was detected in the culture medium of transformed cells using ELISA and Western blot analyses. These data suggested that the human fetal hepatocyte cell line has been established by infection of HBV, in which this cell line secreted viral particles into the culture medium.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)