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Title 

Polychlorinated biphenyls activate caspase-3-like death protease in Vitro but not in Vivo

Authors 

Sang-Han LeeEun Soo YoukYoung Jin JeonSang Bae HanHyoung-Chin KimHwan Mook Kim

Publisher 

Pharmaceutical Society of Japan

Issue Date 

2001

Citation 

Biological & Pharmaceutical Bulletin, vol. 24, no. 12, pp. 1380-1383

Keywords 

caspase-3-like proteasepolychlorinated biphenylserum albuminsplenocytecaspase 3polychlorinated biphenyl derivativein vitro studyin vivo studycaspasescell death

Abstract 

We prove here that serum albumin inhibits apoptosis induced by polychlorinated biphenyls (PCBs), confirming that serum albumin binds to PCB, and that the albumin-PCB complexes inhibit apoptosis in HL-60 cells. We found that PCB (50 μM) increased the activity of caspase-3-like protease when HL-60 cells, as well as splenocytes, were cultured in "serum-free medium." Benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone (Z-VAD-fmk) inhibited apoptosis in cells cultured in the serum-free medium containing 50 μM PCB. To elucidate whether or not PCBs induce apoptosis in vivo, we examined apoptosis of splenocytes by administering PCB to ICR mice (100, 500, 1000 mg·kg-1·d-1) for 5 d and characterizing splenocytes. Interestingly, splenocytes treated with PCB did not show any changes characteristic of apoptosis. These results demonstrate that PCB activates the caspase-3-like death protease in vitro in serum-free medium, but does not induce apoptosis of splenocytes in vivo, suggesting that blood serum may mask the apoptosis induced by PCB.

ISSN 

0918-6158

Link 

http://dx.doi.org/10.1248/bpb.24.1380

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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