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Title 

Molecular cloning of a novel pathogen-inducible cDNA encoding a putative acyl-CoA synthetase from Capsicum annuum L

Authors 

Sang Jik LeeMi Chung SuhShin Je KimJin Kyung KwonMin Woo KimKyung Hee PaekDo Il ChoiByung Dong Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2001

Citation 

Plant Molecular Biology, vol. 46, no. 6, pp. 661-671

Keywords 

acyl-CoA synthetaseAMP-binding proteincapsicum annuum L.mRNA differential displaysalicylic acidsubcellular localizationacyl coenzyme AcDNA clonecomplementary DNAdifferential display

Abstract 

By means of differential display, a pool of salicylic acid (SA)-induced mRNAs were identified and subsequently their cDNAs were isolated from a cDNA library prepared from SA-induced leaf tissues of hot pepper. One of these cDNA clones, designated CaSIG4, was 1900 bp and contained an open reading frame encoding 523 amino acids with a calculated molecular mass of 56.3 kDa. The predicted amino acid sequence of CaSIG4 showed high sequence similarity to the AMP-binding protein family of both prokaryotic and eukaryotic acyl-CoA synthetases. CaSIG4 transcripts accumulated rapidly after SA treatment and in response to both incompatible and compatible interactions with Xanthomonas campestris pv. vesicatoria race 1. To investigate the cis-acting elements mediating CaSIG4 expression, the CaSIG4 5′-flanking region was isolated by inverse PCR. Database searches indicated that a potential cis-regulatory element is almost identical to the consensus core sequences ACC(A/T)ACC(A/C) which are conserved among promoters of other phenylpropanoid biosynthetic genes. The subcellular localization of the CaSIG4 protein was studied by using a soluble modified GFP gene fusion delivered into epidermal cells of onion by biolistic bombardment. The CaSIG4-smGFP fusion protein was localized to the plasma membrane. Taken together, CaSIG4 encoding a putative acyl-CoA synthetase could function as a plasma membrane-bound protein with a role in signaling in plant defense.

ISSN 

0167-4412

Link 

http://dx.doi.org/10.1023/A:1011677028605

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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