상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Conversion of gamma-butyrobetaine to L-carnitine by Achromobacter cycloclast

Authors 

G S NaiduIn Young LeeOck Ki ChoYoung Hoon Park

Publisher 

Springer

Issue Date 

2001

Citation 

Journal of Industrial Microbiology (& Biotechnology), vol. 26, no. 5, pp. 309-315

Keywords 

γ-Butyrobetaineachromobacter cycloclastL-Carnitineproductioncalciumcarnitinegamma butyrobetainenickelpoly(3 hydroxybutyric acid)trace element

Abstract 

L-Carnitine is an ubiquitous substance that plays a major role in the transportation of long-chain fatty acids. We investigated crucial factors that influence microbial conversion of γ-butyrobetaine to L-carnitine using an Achromobacter cycloclast strain. Two-stage culture results showed that γ-butyrobetaine induced enzymes essential for the conversion, which suggests that the precursor should be present in the initial cell growth stage. The addition of yeast extract enhanced L-carnitine production whereas inorganic nitrogen sources inhibited it. Under nitrogen-limiting conditions, the cells accumulated poly-β-hydroxybutyrate instead of L-carnitine. Among the trace elements tested, nickel addition enhanced L-carnitine production by almost twice that of the control and copper strongly inhibited the conversion. L-Carnitine production was reduced when the medium contained inorganic salts of sodium, potassium, and calcium at a concentration greater than 2 g I-1. A higher L-carnitine yield was achieved when cells were incubated in a lower culture volume. The optimal pH for L-carnitine production was 5 to 5.5, whereas that of growth was 7.0, indicating that a pH shift was required. Under optimal conditions, L-carnitine concentrations as high as 15 g I-1 were obtained in 62 h with a 45% molar conversion yield.

ISSN 

0169-4146

Link 

http://dx.doi.org/10.1038/sj.jim.7000135

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)