상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Cloning and characterization of the Mycobacterium bovis BCG panB gene encoding ketopantoate hydroxylmethyltransferase

Authors 

Jin Koo KimKwang Dong KimJong-Seok LimHee Gu LeeSang Jae KimSang Hyun ChoWon Hwa JeongIn Seong ChoeTai Wha ChungSang Gi PaikYong Kyung Choe

Publisher 

Springer Verlag (Germany)

Issue Date 

2001

Citation 

Journal of Biochemistry and Molecular Biology, vol. 34, no. 4, pp. 342-346

Keywords 

BCGKetopantoate hydroxymethyltransferase (KPHMT)panBPantothenic acid

Abstract 

The Mycobacterium bovis BCG panB gene, encoding ketopantoate hydroxymethyltransferase (KPHMT), was cloned from a λgt11 genomic library and sequenced. The DNA sequence encodes a protein that contains 281 amino acid residues (Mr 29,337) with a high similarity to the KPHMTs. Subcloning of a 846 bp open reading frame (ORF), but not a 735 bp ORF, into the vector pUC19 led to complementation of the panB mutant of Escherichia coli. The BCG panB gene was overexpressed in E. coli and the KPHMT purified to homogeneity. The recombinant protein was further confirmed by an enzymatic assay.

ISSN 

1225-8687

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)