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Title 

Levan production by use of the recombinant levansucrase immobilized on titanium-activated magnetite

Authors 

Ki Hyo JangKi Bang SongBuem Seek ParkChul Ho KimBong Hyun ChungRyo Won ChoueKwang Suck LeeChan LeeUck Han ChunSang Ki Rhee

Publisher 

Elsevier

Issue Date 

2001

Citation 

Process Biochemistry, vol. 37, no. 4, pp. 339-343

Keywords 

immobilizationlevanlevansucrasetitanium-activated magnetitezymomonas mobilismagnetiterecombinant enzymetitanium

Abstract 

Levansucrase from Zymomonas mobilis expressed in Escherichia coli was immobilized onto the surface of magnetite. Optimum conditions for the immobilization were, pH 4.0; immobilization reaction time of 30 min and 8 U of enzyme per gram of matrix. Immobilization yield was 75% under optimized conditions. The maximum production yield of levan by the immobilized levansucrase was about 42% on a fructose released basis with sucrose as substrate at 100 g/l. Thermal stability of the levansucrase increased by immobilization procedure. Furthermore, immobilized enzyme showed the less polymerizing activity in levan formation, producing the low-molecular weight (MW) levan. The molecular weight of levan can be controlled by using the immobilized levansucrase in high yield. Immobilized levansucrase retained 61% of the original activity after five repeated uses.

ISSN 

0032-9592

Link 

http://dx.doi.org/10.1016/S0032-9592(01)00215-1

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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