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Title 

Purification and characterization of a novel transfructosylating enzyme from Bacillus macerans EG-6

Authors 

Jong Pil ParkTae Kwang OhJong Won Yun

Publisher 

Elsevier

Issue Date 

2001

Citation 

Process Biochemistry, vol. 37, no. 5, pp. 471-476

Keywords 

bacillus macerans EG-6fructo-oligosaccharidepurificationtransfructosylating enzymeammonium sulfatebacterial enzymefructose oligosaccharidesepharoseunclassified drugbacillus

Abstract 

A novel transfructosylating enzyme, which produces fructo-oligosaccharides from sucrose from Bacillus macerans EG-6 were purified 63.5-fold by ammonium sulphate precipitation (20-60%), CM-Sepharose CL 6B and fast protein liquid chromatographies on Resource Q, Phenyl-Superose HR 5/5 and Mono S (Pharmacia, Uppsala, Sweden). The minimum molecular mass of the purified enzyme was 66 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme was stable at the pH range of 5.0-7.0 and had an optimum pH at 5.0. The optimum temperature for enzyme activity was at 50°C. The oligosaccharide compositions in reaction products were significantly different on using the enzyme obtained from each purification step. For example, crude enzyme unusually produced selectively GF5- and GF6-fructo-oligosaccharide whereas purified enzyme produced mainly 1-kestose (GF2) and nystose (GF3) as in the case of other transfructosylating enzymes.

ISSN 

0032-9592

Link 

http://dx.doi.org/10.1016/S0032-9592(01)00237-0

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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