상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Grb2 dominantly associates with dynamin II in human hepatocellular carcinoma HepG2 cells

Authors 

Sun Young YoonMoon Jin JeongJi Yun YooKyung Im LeeByoung-Mog KwonDo Seon LimChoong Eun LeeYoung Mee ParkMi Young Han

Publisher 

Wiley-Blackwell

Issue Date 

2002

Citation 

Journal of Cellular Biochemistry, vol. 84, no. 1, pp. 150-155

Keywords 

dynamin IIGrb2/SH3 domain-binding proteinshepatocarcinoma HepG2 cellcell proteindynamincancer cell culturecell differentiationhumanhuman cellliver cell carcinoma

Abstract 

The two SH3 domains and one SH2 domain containing adaptor protein Grb2 is an essential element of the Ras signaling pathway in multiple systems. The SH2 domain of Grb2 recognizes and interacts with phosphotyrosine residues on activated tyrosine kinases, whereas the SH3 domains bind to several proline-rich domain-containing proteins such as Sos1. To define the difference in Grb2-associated proteins in hepatocarcinoma cells, we performed coprecipitation analysis using recombinant GST-Grb2 fusion proteins and found that several protein components (p170, p125, pl00, and p80) differently associated with GST-Grb2 proteins in human Chang liver and hepatocarcinoma HepG2 cells. Sos1 and p80 proteins dominantly bind to Grb2 fusion proteins in Chang liver, whereas pl00 remarkably associate with Grb2 in HepG2 cells. Also GST-Grb2 SH2 proteins exclusively bound to the p46Shc, p52Shc, and p66Shc are important adaptors of the Ras pathway in HepG2 cells. The pl00 protein has been identified as dynamin II. We observed that the N-SH3 and C-SH3 domains of Grb2 fusion proteins coprecipitated with dynamin II besides Sos1. These results suggest that dynamin II may be a functional molecule involved in Grb2-mediated signaling pathway on Ras activation for tumor progression and differentiation of hepatocarcinoma cells.

ISSN 

0730-2312

Link 

http://dx.doi.org/10.1002/jcb.1275

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)