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Title 

Internalization of tenecin 3 by a fungal cellular process is essential for its fungicidal effect on Candida albicans

Authors 

Dae Hee KimDong Gun LeeKil Lyong KimYoung Hoon Lee

Publisher 

Federation of European Biochemical Societies

Issue Date 

2001

Citation 

European Journal of Biochemistry, vol. 268, no. 16, pp. 4449-4458

Keywords 

antifungal proteinfungusinsectinternalizationtenecin 3fungal proteinunclassified druganalytic methodartificial membranecandida albicans

Abstract 

Tenecin 3 is a glycine-rich, antifungal protein of 78 residues isolated from the insect Tenebrio molitor larva. As an initial step towards understanding the antifungal mechanism of tenecin 3, we examined how this protein interacts with the pathogenic fungus Candida albicans to exert its antifungal action. Tenecin 3 did not induce the release of a fluorescent dye trapped in the artificial membrane vesicles and it did not perturb the membrane potential of C. albicans by the initial interaction. Fluorescence confocal microscopy and flow cytometric analysis revealed that tenecin 3 is rapidly internalized into the cytoplasmic space in energy-dependent and temperature-dependent manners. This internalization is also dependent on the ionic environment and cellular metabolic states. These results suggest that the internalization of tenecin 3 into the cytoplasm of C. albicans is mediated by a fungal cellular process. The internalized tenecin 3 is dispersed in the cytoplasm, and the loss of cell viability occurs after this internalization.

ISSN 

0014-2956

Link 

http://dx.doi.org/10.1046/j.1432-1327.2001.02364.x

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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