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Title 

Molecular cloning and characterization of Mycobacterium bovis BCG pcp gene encoding pyrrolidone carboxyl peptidase

Authors 

Jin Koo KimSe Jin KimHee Gu LeeJong-Seok LimSang Jae KimSang Hyun ChoWon Hwa JeongIn Seong ChoeTai Wha ChungSang Gi PaikYong Kyung Choe

Publisher 

Springer Verlag (Germany)

Issue Date 

2001

Citation 

Molecules and Cells, vol. 12, no. 3, pp. 347-352

Keywords 

mycobacterium bovis BCGpcp genepyrrolidone carboxyl peptidase (Pcp)5 oxoprolyl peptidasegeneticsmolecular cloningmolecular geneticsmycobacterium boviscloning, molecularmolecular sequence data

Abstract 

The Mycobacterium bovis bacilli Calmette-Guerin (BCG) pcp gene that encodes the pyrrolidone carboxyl peptidase (Pcp) was cloned from a λgt11 genomic library and sequenced. The nucleotide sequence contains a 669 bp open reading frame coding for a protein of 222 amino acid residues with a calculated molecular mass of 23,209 Da. The deduced amino acid sequence is highly homologous to the Pcps from Bacillus amyloliquefaciens, Pseudomonas fluorescens, Bacillus subtilis, Streptococcus pyogenes, and Staphylococcus aureus. A multiple sequence alignment revealed highly conserved domains. The BCG pcp gene was overexpressed in Escherichia coli. The Pcp was purified to homogeneity. The recombinant protein was further confirmed by an enzymatic assay.

ISSN 

1016-8478

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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