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Title 

Removal of 5 '- and 3 '-untranslated regions enhances erythropoietin gene expression in mammalian cells

Authors 

J H ParkC KimW B KimYoung Kook KimS Y LeeJ M Yang

Publisher 

Springer Verlag (Germany)

Issue Date 

2001

Citation 

Molecular Biology +, vol. 35, no. 3, pp. 350-353

Keywords 

ErythropoietinSRα promoterTranscription regulationUntranslated region

Abstract 

The effect of the 5′-and 3′-untranslated regions (UTR) of human erythropoietin gene (gEpo) on its expression in mammalian cells was investigated. 5′-UTR-deleted, 3′-UTR-deleted, and 5′,3′-UTR-deleted gEpos were transfected into COS-7 cells, and the amount of transiently expressed Epo was measured. Cells transfected with the 5′,3′-UTR-deleted gEpo showed the highest expression, 2.6 times that with wild-type gEpo. Northern blot analysis indicated that the enhanced expression of 5′,3′-UTR-deleted gEpo might be related with the transcription efficiency.

ISSN 

0026-8933

Link 

http://dx.doi.org/10.1023/A:1010466526838

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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