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Title 

Identification of total extracellular fibrinase from Bacillus sp. DJ using one- or two- dimensional fibrin Zymography for proteomic approach

Authors 

Nack Shick ChoiJin Young LeeKab Seog YoonKyoung Yoen HanSeung Ho Kim

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2001

Citation 

Journal of Microbiology and Biotechnology, vol. 11, no. 6, pp. 1111-1114

Keywords 

bacillusfibrinaseproteomicstwo-dimensional fibrin zymographyfibrinbacterium identificationextracellular spacespecies differencetwo dimensional gel electrophoresiszymography

Abstract 

An extracellular fibrinolytic-enzyme-producing bacterium was isolated from Doen-Jang, a Korean traditional fermented food, and identified as Bacillus sp. DJ based on its morphology and cellular fatty acid composition. The total extracellular fibrinase (EF) from Bacillus sp. DJ was analyzed using three fibrin zymographic techniques, SDS-fibrin zymography (SDS-FZ), isoelectrofocucing-fibrin zymography (IEF-FZ), and a two-dimensional SDS-fibrin zymographic analysis (2D SDS-FZ). As a result, the EF map of Bacillus sp. DJ was established. The results suggest that the 2D SDS-FZ method will be a useful tool for the proteomic approach for many other bacterial proteases.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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