상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

The human elongation factor 1 alpha (EF-1α) first intron highly enhances expression of foreign genes from the murine cytomegalovirus promoter

Authors 

Seon-Young KimJae Ho LeeHyun Seock ShinHye Ja KangYeon Soo Kim

Publisher 

Elsevier

Issue Date 

2002

Citation 

Journal of Biotechnology, vol. 93, no. 2, pp. 183-187

Keywords 

enhancererythropoietinhuman elongation factor one alpha first intronMCMV IE promoterplasmidgeneshuman engineeringmammalian expression vectorgenetic engineeringelongation factor 1alpha

Abstract 

In order to develop a highly efficient mammalian expression vector, we constructed a vector by the combination of the murine cytomegalovirus (MCMV) immediate early (IE) promoter and the human elongation factor one alpha (EF-1α) first intron. The MCMV IE promoter was several fold stronger than the human cytomegalovirus (HCMV) immediate early (IE) promoter and the human elongation factor one alpha (EF-1α) promoter in various mammalian cell lines such as NIH3T3, Neuro-2a, 293T or HT1080 and was only slightly weaker than the HCMV or the EF-1α promoter in HeLa and CHO cell lines. We inserted the first intron of the human EF-1α gene behind the MCMV and the HCMV promoter to enhance the gene expression through increasing RNA transcription and/or RNA stability. The insertion of the human EF-1α first intron markedly enhanced the level of gene expression in many cell lines and the resultant MCMV promoter with the human EF-1α first intron (MCMV/EF-I) was higher than the HCMV promoter from 4.3 to 65.5-fold in various cell lines. Also, the MCMV/EF-I promoter induced higher level of erythropoietin expression than the HCMV promoter in transiently transfected CHO cells.

ISSN 

0168-1656

Link 

http://dx.doi.org/10.1016/S0168-1656(01)00388-1

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)