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Title 

Protein phosphatase 2A modulates the proliferation of human multiple myeloma cells via regulation of the production of reactive oxygen intermediates and anti-apoptotic factors

Authors 

Hyung Sik KangIn Pyo Choi

Publisher 

Elsevier

Issue Date 

2001

Citation 

Cellular Immunology, vol. 213, no. 1, pp. 34-44

Keywords 

Apoptosisbcl-2IL-6myelomaPP2AROIphosphoprotein phosphatase 2Aprotein bcl 2reactive oxygen metabolitecell survival

Abstract 

To understand the roles of reactive oxygen intermediates (ROI) in Fas-mediated apoptosis of myeloma cells, the effects of antioxidants were tested. Fas-mediated apoptosis was further increased in the presence of antioxidants such as N-acetyl-L-cysteine and glutathione, but it was decreased when hydrogen peroxide was added. The intracellular ROI level was significantly decreased in myeloma cells treated with okadaic acid, an inhibitor of protein phosphatases 1 and 2A (PP1/PP2A). To clarify the direct roles of PP2A in myeloma cell growth, the PP2A transfected cell lines, sense- or antisense-PP2A transfectants, were established. Spontaneous cell growth of antisense-PP2A transfectants was reduced compared with that of vector transfectants. The intracellular ROI level was significantly decreased in antisense-PP2A transfectants but increased in sense-PP2A transfectants compared with vector controls. In addition, anti-apoptotic factors such as bcl-2 and IL-6 were reduced in antisense-PP2A transfectants. Taken together, these results indicate that PP2A is an essential factor for survival and growth of myeloma cells via regulation of intracellular ROI and anti-apoptotic factors.

ISSN 

0008-8749

Link 

http://dx.doi.org/10.1006/cimm.2001.1861

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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