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Title 

Complestatin antagonizes the AMPA/Kainate-induced neurotoxicity in cultured chick telencephalic neurons

Authors 

Ick Dong YooBong Sik YunIn Ja RyooSoo Young LeeMyeong Heon ShinSei Kwan Oh

Publisher 

Springer Verlag (Germany)

Issue Date 

2002

Citation 

Neurochemical Research, vol. 27, no. 4, pp. 337-343

Keywords 

AMPA/kainitechick neuroncomplestatinneurotoxicityAMPA receptor antagonistchick embryocells, cultured

Abstract 

Excitatory amino acids are known to induce considerable neurotoxicity in central nervous system. In the present study, the neurotoxicity was induced by application of kainate or AMPA in chick telencephalic neuron, and neuroprotective activity was tested with complestatin that was isolated from streptomyces species. In cultured telencephalic neurons exposed to 500 μM kainate for 2 days, the AMPA/kainate receptor antagonist 6,7-dinitroquinoxaline-2,3-dione (DNQX, 5 μM) completely blocked kainate-induced neurotoxicity. Also, complestatin (0.5 μM) completely blocked kainate-induced neuronal injury at a concentration lower than that required for prototype AMPA/kainate receptor antagonist DNQX. In addition, complestatin blocked AMPA-induced neurotoxicity when the neurons were pretreated with cyclothiazide, a desensitization blocker of AMPA receptor. Surprisingly, when the onset of the treatment was delayed for 6 hours, complestatin led to a reduction in kainate-induced neuronal injury. While inhibition of protein kinase C (PKC) by staurosporin induced neurotoxicity, that was blocked by complestatin. Activation of PKC by phorbol dibutyrate partially inhibited the kainate-induced neurotoxicity. These results suggest that complestatin may be used as an anti-excitotoxic agent and involved in the PKC activation contributing to inhibition of neurotoxicity.

ISSN 

0364-3190

Link 

http://dx.doi.org/10.1023/A:1014919531306

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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