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Title 

Limited demethylation leaves mosaic-type methylation states in cloned bovine pre-implantation embryos

Authors 

Yong Kook KangJung Sun ParkDeog Bon KooYoung Hee ChoiSun-Uk KimKyung Kwang LeeYong Mahn Han

Publisher 

Nature Publishing Group

Issue Date 

2002

Citation 

EMBO Journal, vol. 21, no. 5, pp. 1092-1100

Keywords 

bovinecloningdemethylationepigenetic reprogrammingpre-implantation embryosDNA methylationembryopreimplantation embryoDNA Methylation

Abstract 

Cloning by nuclear transfer (NT) has been riddled with difficulties: most clones die before birth and survivors frequently display growth abnormalities. The cross-species similarity in abnormalities observed in cloned fetuses/animals leads us to suspect the fidelity of epigenetic reprogramming of the donor genome. Here, we found that single-copy sequences, unlike satellite sequences, are demethylated in pre-implantation NT embryos. The differential demethylation pattern between genomic sequences was confirmed by analyzing single blastocysts. It suggests selective demethylation of other developmentally important genes in NT embryos. We also observed a reverse relationship between methylation levels and inner cell mass versus trophectoderm (ICM/TE) ratios, which was found to be a result of another type of differential demethylation occurring in NT blastocysts where unequal methylation was maintained between ICM and TE regions. TE-localized methylation aberrancy suggests a widespread gene dysregulation in an extra-embryonic region, thereby resulting in placental dysfunction familiar to cloned fetuses/animals. These differential demethylations among genomic sequences and between differently allocated cells produce varied overall, but specified, methylation patterns, demonstrating that epigenetic reprogramming occurs in a limited fashion in NT embryos.

ISSN 

0261-4189

Link 

http://dx.doi.org/10.1093/emboj/21.5.1092

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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