상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Activation of dynamin I gene expression by Sp1 and Sp3 is required for neuronal differentiation of N1E-115 cells

Authors 

Ji Yun YooMoon Jin JeongByoung-Mog KwonMan Wook HurYoung Mee ParkMi Young Han

Publisher 

American Society for Biochemistry and Molecular Biology

Issue Date 

2002

Citation 

Journal of Biological Chemistry, vol. 277, no. 14, pp. 11904-11909

Keywords 

cellsgenesmutagenesisproteinsgel shift analysisneuronal differentiationbiochemistrydynaminimmunoglobulin enhancer binding proteinmithramycin

Abstract 

Dynamin I is a key molecule required for the recycling of synaptic vesicles in neurons, and it has been known that dynamin I gene expression is induced during neuronal differentiation. Our previous studies established that neuronal restriction of dynamin I gene expression is controlled by Spl and nuclear factor-KB-like element-1. Here, using a series of deletion constructs and site-directed mutation, we found that transcription of dynamin I gene during neuronal differentiation of N1E-115 cells is controlled primarily by the Spl element located between -13 to -4 bp of the dynamin I promoter. Gel shift analysis demonstrated that in addition to SpI, Sp3 could interact with this Spl element. The requirement for Sp family transcription factors in dynamin I gene expression was confirmed by using mithramycin, an inhibitor of Sp1/Sp3 binding. Mithramycin repressed dynamin I gene expression and resulted in blocking of neuronal differentiation of NIE-115 cells. The localization of the dynamin I protein was also restricted in the peripheral region of the nucleus by the mithramycin treatment. Thus, all of our results suggest that induction of dynamin I gene expression during N1E-115 cell differentiation is modulated by Sp1/Sp3 interactions with the dynamin I promoter, and its expression is important for neuronal differentiation of the N1E-115 cells.

ISSN 

0021-9258

Link 

http://dx.doi.org/10.1074/jbc.M111788200

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)