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Title 

Relationship between acrylamide concentration and enzymatic activity in an improved single fibrin zymogram gel system

Authors 

Nack Shick ChoiByoung Young KimJin Young LeeKab Seog YoonKyoung Yoen HanSeung Ho Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2002

Citation 

Journal of Biochemistry and Molecular Biology, vol. 35, no. 2, pp. 236-238

Keywords 

bacillusextracellular proteasefibrin zymographyacrylamidefibrinfibrinolysispolyacrylamide gel electrophoresiselectrophoresis, polyacrylamide gel

Abstract 

Based on the zymography analysis, Bacillus sp. DJ-4 (screened from Doen-Jang, a Korean traditional fermented food) secretes seven extracellular fibrinolytic enzymes (EFEs; 68, 64, 55, 45, 33, 27, and 13 kDa) in culture broth. These seven EFEs were analyzed by newly applied SDS-fibrin zymography combined with gradient polyacrylamide (SDS-FZGP). This improved gel system was used with a 5-20% acrylamide gradient in a fibrin zymogram gel for the separation of proteins with molecular masses from below 10 kDa to over 100 kDa on one gel plate. Using this system, high molecular weight bands (HMWBs) were clearly and sharply resolved. We also examined the relationship between an acrylamide concentration and the enzymatic activity of EFE using densitometric analysis.

ISSN 

1225-8687

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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