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Title 

Prometastatic effect of N-acetylglucosaminyltransferase V is due to modification and stabilization of active matriptase by adding β1-6 GlcNAc branching

Authors 

Shinji IharaEiji MiyoshiJeong Heon KoKohei MurataSusumu NakaharaKoichi HonkeRobert B DicksonChen Yong LinNaoyuki Taniguchi

Publisher 

American Society for Biochemistry and Molecular Biology

Issue Date 

2002

Citation 

Journal of Biological Chemistry, vol. 277, no. 19, pp. 16960-16967

Keywords 

activation analysisbiodegradationmalignant transformationsenzymesglycoproteinmannosidematriptasen acetylglucosaminyltransferasephytohemagglutininunclassified drug

Abstract 

Oligosaccharide moieties of glycoproteins are structurally altered during development, carcinogenesis, and malignant transformations. It is well known that β1-6 GlcNAc branching, a product of UDP-GlcNAc α-mannoside β1-6-N-acetylglucosaminyltransferase (GnT-V), is associated with malignant transformation as the results of such alterations. However, the mechanism by which β1-6 GlcNAc branching is linked to metastasis remains unclear, because the identification of specific glycoprotein(s) that are glycosylated by GnT-V and its biological function have not been examined. We herein report that matriptase, which activates both urokinase-type plasminogen activator and hepatocyte growth factor, is a target protein for GnT-V. The overexpression of GnT-V in gastric cancer cells leads to severe peritoneal dissemination in athymic mice, which can be attributed to the increased expression of matriptase. This increase was due to the acquired resistance of matriptase to degradation, since it is glycosylated by GnT-V and a corresponding increase in the active form. These results indicate that this process is a key element in malignant transformation, as the direct result of oligosaccharide modification.

ISSN 

0021-9258

Link 

http://dx.doi.org/10.1074/jbc.M200673200

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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