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Title 

Glycolipid biosurfactants produced by Pseudomonas aeruginosa D2D2 from diesel-contaminated soil

Authors 

Hye Joon MoonYoung Kuong LimHee Sik KimDae Young KwonWook Jin Chung

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2002

Citation 

Journal of Microbiology and Biotechnology, vol. 12, no. 3, pp. 371-376

Keywords 

biosurfactantglycolipidpseudomonas aeruginosa D2D2rhamnolipiddiesel fuelpseudomonas aeruginosasoil pollution

Abstract 

A biosurfactant-producing bacterial strain was selected from diesel-contaminated soil by measuring the oil-film collapsing activity and identified as Pseudomonas aeruginosa D2D2. When glucose and olive oil were used as carbon sources, 11.46 g/l of biosurfactant was obtained. Based on TLC analysis, the biosurfactant produced from P. aeruginosa D2D2 was identified as a glycolipid, consisting of two types of biosurfactants (Type I and Type II). The purified glycolipid reduced the surface tension of the culture from 72 dyne/cm to 27 dyne/cm. The hydrophilic and hydrophobic moiety of the biosurfactant were rhamnose and β-hydroxydecanoic acid, as determined by FAB-MS and NMR analyses, respectively.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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