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Title 

A new spin filter for high density culture and ethanol production by Saccharomyces cerevisiae

Authors 

Hyun Soo MoonDong Joon LimGu Young SongHyun Ah KangSeung Wook KimIk Hwan KimSuk In Hong

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2002

Citation 

Journal of Microbiology and Biotechnology, vol. 12, no. 3, pp. 406-410

Keywords 

centrifugal forcehigh density culturepivotal movementspin filteryeastalcoholstainless steelbioreactorcell separationconcentration (parameters)

Abstract 

A new spin filter consisting of 50 μm (nominal pore size) depth filters rolled on a stainless steel grid was developed, using Saccharomyces cerevisiae as a model suspension cell to evaluate the spin filter performance. In a 1.8-1 fermentor with a rotation speed of 300 rpm and perfusion rate of 4 ml/min, a cell concentration of 49 g/l and ethanol concentration of 45 g/l from 100 g/l glucose could be obtained in a perfusion culture. The major mechanisms for cell separation used by the large-pore spin filter appeared to be centrifugal force and pivotal movement of the cells in the spin filter.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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