상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Isolation and biological properties of novel cell cycle inhibitors, HY558, isolated from Penicillium minioluteum F558

Authors 

Chul Hoon LeeHae Young LimMin Kyoung KimYoul Hee ChoDeok Kun OhChang Jin KimYoung Ho Lim

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2002

Citation 

Journal of Microbiology and Biotechnology, vol. 12, no. 3, pp. 470-475

Keywords 

cell cycle inhibitorHY558penicillium minioluteum F558cyclin dependent kinase inhibitorhy558bacterium isolationcancer cell culturecell cycle G1 phasecell cycle G2 phasecell proliferation

Abstract 

In the course of screening for a novel cell cycle inhibitor, a potent Cdk 1 inhibitor, HY558, was found from the culture broth of Penicillium minioluteum F558 isolated from a soil sample. The molecular ion of HY558 was identified at m/z 329 (MH+) with a molecular formula of C20H44ON2. HY558 exhibited selective antiproliferative effects on various human cancer cell lines. Its IC50 values were estimated to be 0.29 mM on HepG2, 0.30 mM on HeLa, 0.30 mM on HL60, 0.33 mM on HT-29, and 0.25 mM on AGS cells. Interestingly, HY558 demonstrated no antiproliferative effect with normal lymphocytes used as the control, and a low level of inhibition on the proliferation of A549 cancer cells. A flow cytometric analysis of HepG2 cells revealed an appreciable arrest of cells at the G1 and G2/M phases of the cell cycle following treatment with HY558. Furthermore, DNA fragmentation due to apoptosis was observed in HeLa cells treated with 0.46 mM of HY558.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)