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Title 

Carnitine uptake by AGP2 in yeast Saccharomyces cerevisiae is dependent on Hog1 MAP kinase pathway

Authors 

Ji Young LeeBo Young LeeDong Jin ShinSang Soo KwakJeong Dong BahkChae Oh LimDae Jin Yun

Publisher 

Springer Verlag (Germany)

Issue Date 

2002

Citation 

Molecules and Cells, vol. 13, no. 3, pp. 407-412

Keywords 

carnitine uptakeexpressionHOG Pathwayosmotic stressS. cerevisiaecarnitinemitogen activated protein kinaseprotein Hog1Agp2 protein, S cerevisiaeHOG1 protein, S cerevisiae

Abstract 

The AGP2 gene encodes a plasma membrane carnitine transporter in S. cerevisiae. Here, we report the identification of AGP2 as an osmotic stress response gene. AGP2 was isolated from mTn3 tagged mutants that contained in-frame fusions with lacZ. The expression of AGP2 was down-regulated by osmotic stresses, including NaCl, sorbitol, and KCl. We also found that carnitine uptake was inhibited by NaCl. In the ssk1Δ ste11Δ double-mutant strain, the expression of AGP2 and the uptake of carnitine were greatly reduced compared to the wild-type strain. Furthermore, carnitine uptake was inhibited by the constitutive expression of PBS2, which encodes a MAPKK that activates Hog1. We concluded, therefore, that the HOG pathway plays an important role in the regulation of carnitine uptake in S. cerevisiae.

ISSN 

1016-8478

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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