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Title 

Novel high-level constitutive expression system, pHCE vector, for a convenient and cost-effective soluble production of human tumor necrosis factor-α

Authors 

Ha Ryoung PooJae Jun SongSeung Pyo HongYoon Ho ChoiSun Woo YunJin Ho KimSang Chul LeeSeung Goo LeeMoon Hee Sung

Publisher 

Springer Verlag (Germany)

Issue Date 

2002

Citation 

Biotechnology Letters, vol. 24, no. 14, pp. 1185-1189

Keywords 

constitutive expressionisopropyl-beta-D-thiogalactosidasePHCErecombinant human tumor necrosis factoraminotransferaserecombinant tumor necrosis factor alphatumor necrosis factor alphacomparative studycost effectiveness analysiscytokine production

Abstract 

A new E. coli expression system, the pHCE-IIB vector, using a constitutively expressing HCE promoter derived upstream from the D-amino acid aminotransferase (D-AAT) gene of Geobacillus toebii, was developed for the high-level expression of foreign proteins without induction. The expression efficiency of recombinant human tumor necrosis factor-α (rhTNFα) using pHCE-IIB was compared with that of the pET14B vector under the control of a T7 promoter induced by IPTG. The total amount of rhTNFα produced by the pHCE system was approximately twice that produced by the pET system under optimal fermentation conditions thereby demonstrating the convenience and economical advantage of the new constitutive pHCE-IIB expression vector over the conventional pET expression vector.

ISSN 

0141-5492

Link 

http://dx.doi.org/10.1023/A:1016107230825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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