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Title 

Human lactoferrin activates transcription of IL-1β gene in mammalian cells

Authors 

K N SonJ B ParkC K ChungD K ChungDae Yeul YuKyung Kwang LeeJ Y Kim

Publisher 

Elsevier

Issue Date 

2002

Citation 

Biochemical and Biophysical Research Communications, vol. 290, no. 1, pp. 236-241

Keywords 

cytokineDNA binding domainIL-1βlactoferrintransactivationcell DNAinterleukin 1betaphorbol 13 acetate 12 myristateanimal cell

Abstract 

Lactoferrin (Lf) has been suggested to play roles in primary defense against microbial infection and other cellular processes including immunomodulation. Lf is known to bind to DNA and implicated to activate transcription. In the present study, we demonstrated that Lf stimulated transcription of IL-1β gene, one of natural genes containing putative Lf binding site (LBS) in the 5′-flanking sequences. K562 cells treated with a combination of Lf and PMA showed a synergistic induction in the level of IL-1β mRNA over treatment with PMA alone. Synergistic stimulation of IL-1β expression by Lf and PMA was also confirmed by IL-1β/Luc reporter gene assays. Analysis of Lf domains revealed that the transcriptional domain of Lf is located within the N-terminal 90 amino acids, termed NIa and that the C-terminal half lobe lacked the transactivating activity. The NIa, the N-terminal half lobe as well as intact Lf stimulated transcription of IL-1β gene in the transfected K562 cells along with PMA, while the C-terminal half lobe did not. Our results suggest that Lf may play some roles in transcription of IL-1β gene and may also regulate transcription of other natural genes containing LBS.

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1006/bbrc.2001.6181

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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