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Title 

Enhanced tolerances of transgenic tobacco plants expressing both superoxide dismutase and ascorbate peroxidase in chloroplasts against methyl viologen-mediated oxidative stress

Authors 

Suk Yoon KwonYu Jeong JeongHeang Soon LeeJ S KimK Y ChoR D AllenSang Soo Kwak

Publisher 

Wiley-Blackwell

Issue Date 

2002

Citation 

Plant, Cell and Environment, vol. 25, no. 7, pp. 873-882

Keywords 

antioxidant enzymeleaf discmembrane damagenicotiana tabacum tobaccostress tolerancewhole plantenvironmental stresstobaccotolerancetransgenic plant

Abstract 

In order to better understand the role of antioxidant enzymes in plant stress protection mechanisms, transgenic tobacco (Nicotiana tabacum cv. Xanthi) plants were developed that overexpress both superoxide dismutase (SOD) and ascorbate peroxidase (APX) in chloroplasts. These plants were evaluated for protection against methyl viologen (MV, paraquat)-mediated oxidative damage both in leaf discs and whole plants. Transgenic plants that express either chloroplast-targeted CuZnSOD (C) or MnSOD (M) and APX (A) were developed (referred to as CA plants and AM plants, respectively). These plant lines were crossed to produce plants that express all three transgenes (CMA plants and AMC plants). These plants had higher total APX and SOD activities than non-transgenic (NT) plants and exhibit novel APX and SOD isoenzymes not detected in NT plants. As expected, transgenic plants that expressed single SODs showed levels of protection from MV that were only slightly improved compared to NT plants. The expression of either SOD isoform along with APX led to increased protection while expression of both SODs and APX provided the highest levels of protection against membrane damage in leaf discs and visual symptoms in whole plants.

Citation 

Plant, Cell and Environment, 25(7): 873-882

ISSN 

0140-7791

Link 

http://dx.doi.org/10.1046/j.1365-3040.2002.00870.x

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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