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Title 

Isolation and characterization of a strain of Bacillus thuringiensis ssp. kurstaki containing a new δ-endotoxin gene

Authors 

M S LiY H JeI H LeeJ H ChangJ Y RohH S KimHyun Woo OhK S Boo

Publisher 

Springer Verlag (Germany)

Issue Date 

2002

Citation 

Current Microbiology, vol. 45, no. 4, pp. 299-302

Keywords 

antiserumbacillus thuringiensis toxinbacterial proteinbacterial toxincryIX protein, bacillus thuringiensisendotoxininsecticidal crystal protein, bacillus thuringiensisbacillus thuringiensisbacterium isolationnonhuman

Abstract 

A strain of Bacillus thuringiensis that showed significantly high toxicity to Plutella xylostella and Spodoptera exigua was isolated from a Korean soil sample and characterized. The isolate, named B. thuringiensis K1, was determined to belong to ssp. kurstaki (H3a3b3c) type by an H antisera agglutination test and produced bipyramidal inclusions. Plasmid pattern of K1 was different from that of the reference strain, ssp. kurstaki HD-1, but the parasporal inclusion protein profile of K1 had two major bands that were similar in size to those of ssp. kurstaki HD-1. To verify the δ-endotoxin gene types of K1, PCR analysis with specific cry gene primers was performed to show that K1 contained a new cry gene in addition to cry1Aa, cry1Ab, cry1Ac, cry1E and cry2 genes. PCR-amplified region of the new cry gene, cpyX, showed 79% similarity to cry1Fa1 gene (GenBank Accession No. M63897). In an insect toxicity assay, K1 had higher toxicity against Plutella xylostella and S. exigua than ssp. kurstaki HD-1.

ISSN 

0343-8651

Link 

http://dx.doi.org/10.1007/s00284-002-3755-0

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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