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Title 

GSH2, a gene encoding γ-glutamylcysteine synthetase in the methylotrophic yeast Hansenula polymorpha

Authors 

V M UbiyvovkT Y NazarkoO G StasykMin Jeong SohnHyun Ah KangA A Sibirny

Publisher 

Wiley-Blackwell

Issue Date 

2002

Citation 

FEMS Yeast Research, vol. 2, no. 3, pp. 327-332

Keywords 

γ-glutamylcysteine synthetaseglutathione biosynthesishansenula polymorphamethylotrophic yeastglutamate cysteine ligaseglutathioneglutathione synthasemethanolcandida albicanscell proliferation

Abstract 

The GSH2 gene, encoding Hansenula polymorpha γ-glutamylcysteine synthetase, was cloned by functional complementation of a glutathione (GSH)-deficient gsh2 mutant of H. polymorpha. The gene was isolated as a 4.3-kb XbaI fragment that was capable of restoring GSH synthesis, heavy-metal resistance and cell proliferation when introduced into gsh2 mutant cells. It possesses 53% identical and 69% similar amino acids compared with the Candida albicans homologue (Gcs1p). In comparison to the Saccharomyces cerevisiae homologue (Gsh1p), it possesses 47% identical and 61% similar amino acids. The GSH2 sequence appears in the GenBank database under accession No. AF435121.

ISSN 

1567-1356

Link 

http://dx.doi.org/10.1016/S1567-1356(02)00111-3

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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