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Title 

Amplification of mitochondrial small subunit ribosomal DNA of polypores and its potential for phylogenetic analysis

Authors 

Soon Gyu HongWon Jin JeongH S Jung

Publisher 

Mycological Society of America

Issue Date 

2002

Citation 

Mycologia, vol. 94, no. 5, pp. 823-833

Keywords 

ganodermahymenomycetesinformation contentuniversal primersmitochondrial DNAphylogenetics

Abstract 

There has been a systematic need to seek adequate phylogenetic markers that can be applied in phylogenetic analyses of fungal taxa at various levels. The mitochondrial small subunit ribosomal DNA (mt SSU rDNA) is generally considered to be one of the molecules that are appropriate for phylogenetic analyses at a family level. In order to obtain universal primers for polypores of Hymenomycetes, mt SSU rRNA genes were cloned from Bjerkandera adusta, Ganoderraa lucidum, Phlebiopsis gigantea, and Phellinus laevigatus and their sequences were determined. Based on the conserved sequences of cloned genes from polypores and Agrocybe aegerita, PCR primers were designed for amplification and sequencing of mt SSU rDNAs. New primers allowed effective amplification and sequencing of almost full-sized genes from representative species of polypores and related species. Phylogenetic relationships were resolved quite efficiently by mt SSU rDNA sequences, and they proved to be more useful in phylogenetic reconstruction of Ganoderma than nuclear internal transcribed spacer (ITS) rDNA sequences.

ISSN 

0027-5514

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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