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Title 

Introduction of hog cholera virus gene into potato plants by Agrobacterium-mediated transformation and the analysis of its expression

Authors 

Hyun Soon KimJae Heung JeonC J KimHyouk Joung

Publisher 

Korea Society of Plant Biotechnology

Issue Date 

2002

Citation 

Journal of Plant Biotechnology, vol. 4, no. 4, pp. 155-161

Keywords 

edible vaccineplant-derived antigengene expression and transgenic potato

Abstract 

The HCV gene was expressed in potato plants under the control of the constitutive CaMV 35S promoter or tuber-specific patatin promoter. Solanum tuberosum plants carrying a plant expression vector harboring the encoding region of HCV gene were generated by Agrobacterium tumefaciens-mediated in vitro transformation methods. The presence of HCV gene in the plant genome was detected by PCR and DNA hybridization experiments. We obtained the 5 lines of transgenic potato with the pMBPHCV construct and 4 lines of transgenic potato with the pATHCV construct. The HCV transgene stably integrated into the potato genome, as well as their transcription. HCV mRNA was identified in leaf and tuber tissues of transgenic plants by Northern blot analysis. The transgenic potato plants produced the expected transcript, and the corresponding HCV protein accumulated in individual transgenic plants.

ISSN 

1229-2818

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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