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Title 

Characterization of extracellular α-galactosidase produced by Streptomyces sp. YB-4

 

균체외 α-galactosidase를 생산하는 Streptomyces sp. YB-4의 분리 및 효소 특성

Authors 

S Y KimK H ChoChang Jin KimDong Jin ParkK H Yoon

Publisher 

The Korean Society for Applied Microbiology and Biotechnology

Issue Date 

2002

Citation 

Korean Journal of Microbiology & Biotechnology, vol. 30, no. 4, pp. 332-338

Keywords 

α-galactosidaseidentificationpropertyreaction productstreptomycesalpha galactosidasestreptomyces sp.

Abstract 

A strain YB-4 producing the extracellular α-galactosidase was isolated from soil, and has been identified as Streptomyces sp. on the basis of its cultural, morphological and physiological properties. The partially purified α-galactosidase was most active on paranitrophenyl-α -D-galactopyranoside at pH 6.0 and 60°C. The enzyme retained 90% of its maximum activity between pH 4.0 and pH 10.0 after pre-incubation for 1 h. The enzyme was able to hydrolyze oligomeric substrates such as melibiose, raffinose and stachyose to liberate galactose residue, indicating that the α-galactosidase of Steptomyces sp. YB-4 hydrolyzed α-1,6 linkage.

ISSN 

0257-2389

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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