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Title 

High frequency somatic embryogenesis and plant regeneration in zygotic embryo cultures of Japanese honeysuckle

Authors 

Suk Weon KimSeung Cheol OhDong Su InJang Ryol Liu

Publisher 

Springer Verlag (Germany)

Issue Date 

2003

Citation 

Plant Cell Tissue and Organ Culture, vol. 72, no. 3, pp. 277-280

Keywords 

caprifoliaceaecell suspension culturesembryogenic calluslonicera japonicasomatic embryoplant cell cultureregenerationplants (botany)

Abstract 

Japanese honeysuckle plant (Lonicera japonica Thunb.) is rich in iridoid secologanin and is a potentially useful model for the study of secologanin biosynthesis. Culture conditions for high frequency plant regeneration via somatic embryogenesis from zygotic embryo cultures and zygotic embryo-derived embryogenic cell suspension cultures of this species are described. Mature zygotic embryos formed embryogenic calluses at a frequency of 46.7% when cultured on Murashige and Skoog (MS) medium supplemented with 4.52 μM 2,4-dichloro-phenoxyacetic acid (2,4-D). Cell suspension cultures were established with embryogenic calluses using liquid MS medium with 4.52 μM 2,4-D. Upon plating onto MS basal medium, embryogenic cell suspension cultures produced numerous somatic embryos, which subsequently developed into plantlets at a frequency of 68%. Regenerated plantlets were transplanted to potting soil and grown to maturity in a greenhouse.

ISSN 

0167-6857

Link 

http://dx.doi.org/10.1023/A:1022360115275

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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