상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Functional over-expression of the Stm1 protein, a G-protein-coupled receptor, in Schizosaccharomyces pombe

Authors 

Kyung Sook ChungDong Uk KimSung Woo RyooEun Jung KangMi Sun WonLi La KimYoung Joo JangP J MaengS C KimHyang Sook YooKwang Lae Hoe

Publisher 

Springer Verlag (Germany)

Issue Date 

2003

Citation 

Biotechnology Letters, vol. 25, no. 3, pp. 267-272

Keywords 

g-protein-coupled receptorHis6 taghomologous expressionschizosaccharomyces pombeseven transmembrane proteinguanine nucleotide binding proteinmembrane proteinrecombinant proteinschizosaccharomyces pombe proteinStm1 protein, S pombe

Abstract 

We report here the first functional over-expression of the Stm1 protein, a G-protein-coupled receptor with seven-trans-membrane spanning regions, in a homologous expression system without internal modification of the open reading frame of Stm1. The entire coding sequence, except for the termination codon followed by a C-terminal His6 tag, has been cloned into the pREP1 vector. The functionally active Stm1-His6 was over-expressed in Schizosaccharomyces pombe under the control of the nmt1 (no message in thiamine) promoter. The expression after induction was 120 times as much as that of control before induction and it gave ∼500 ng protein/2 × 107cells.

ISSN 

0141-5492

Link 

http://dx.doi.org/10.1023/A:1022355102192

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)