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Title 

Coexpression of BiP increased antithrombotic hirudin production in recombinant Saccharomyces cerevisiae

Authors 

M D KimK C HanHyun Ah KangSang Ki RheeJ H Seo

Publisher 

Elsevier

Issue Date 

2003

Citation 

Journal of Biotechnology, vol. 101, no. 1, pp. 81-87

Keywords 

BiPGAL10 promoterhirudinsaccharomyces cerevisiaesecretioncoexpressionhirudinsrecombinant proteins

Abstract 

In order to increase a production level of antithrombotic hirudin, BiP was simultaneously expressed in recombinant Saccharomyces cerevisiae strains carrying ten and 15 copies of the hirudin expression cassette integrated in the chromosome. Coexpression of BiP greatly enhanced both cell growth and hirudin production in recombinant S. cerevisiae. Maximum hirudin concentration of 36 mg l-1 was obtained from batch culture of the ten copy-number transformant concomitantly harboring an episomal copy of the BiP gene under the control of the GAL1 promoter, which is corresponding to a 2.5-fold increase compared with the control strain carrying the genomic BiP gene only. The mean size of the recombinant yeast cells expressing the BiP gene remained at a relatively constant level compared with the control strains of which size increased after the onset of hirudin expression by the GAL10 promoter.

ISSN 

0168-1656

Link 

http://dx.doi.org/10.1016/S0168-1656(02)00288-2

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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