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Title 

The tetrameric structure of Haemophilus influenza hybrid Prx5 reveals interactions between electron donor and acceptor proteins

Authors 

Seung-Jun KimJoo Rang WooY S HwangDae Gwin JeongDong Hae ShinKang Hwa KimSeong Eon Ryu

Publisher 

American Society for Biochemistry and Molecular Biology

Issue Date 

2003

Citation 

Journal of Biological Chemistry, vol. 278, no. 12, pp. 10790-10798

Keywords 

crystal structureproteinshybrid proteinbacterial proteinproteinelectron transporthaemophilus influenzaeprotein protein interactionprotein secondary structure

Abstract 

Cellular redox control is often mediated by oxidation and reduction of cysteine residues in the redox-sensitive proteins, where thioredoxin and glutaredoxin (Grx) play as electron donors for the oxidized proteins. Despite the importance of protein-protein interactions between the electron donor and acceptor proteins, there has been no structural information for the interaction of thioredoxin or Grx with natural target proteins. Here, we present the crystal structure of a novel Haemophilus influenza peroxiredoxin (Prx) hybrid Prx5 determined at 2.8-? resolution. The structure reveals that hybrid Prx5 forms a tightly associated tetramer where active sites of Prx and Grx domains of different monomers interact with each other. The Prx-Grx interface comprises specific charge interactions surrounded by weak interactions, providing insight into the target recognition mechanism of Grx. The tetrameric structure also exhibits a flexible active site and alternative Prx-Grx interactions, which appear to facilitate the electron transfer from Grx to Prx domain. Differences of electron donor binding surfaces in Prx proteins revealed by an analysis based on the structural information explain the electron donor specificities of various Prx proteins.

ISSN 

0021-9258

Link 

http://dx.doi.org/10.1074/jbc.M209553200

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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