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Title 

Isolation of nitrogen-fixing bacteria from gramineous crops and measurement of nitrogenase activity

 

벼과식물로부터 질소고정균의 분리와 Nitrogenase 활성 측정

Authors 

E H ChoiS E LeeK S YoonD K KwonJ K SohnSeung Hwan ParkM S HanS Y Ghim

Publisher 

The Korean Society for Applied Microbiology and Biotechnology

Issue Date 

2003

Citation 

Korean Journal of Microbiology & Biotechnology, vol. 31, no. 1, pp. 18-24

Keywords 

16S rDNA sequencecellulasenitrogen-fixing bacterianitrogenasebacterial genebacteriumbacterium colonybacterium isolationenzyme activitynitrogen fixation

Abstract 

For researching nitrogen-fixing bacteria associated with gramineous crops, we collected growing roots of rices, wheats, oats, barleys, ryes, and maizes at 19 sites of southern Korean peninsula. Endophytes and free living bacteria were isolated from those crop roots. Sixty-three isolates were classified on the basis of different morphology, size, color, host of colony, and the 16S rDNAs sequence. The analyses of PCR amplification for nifH gene and nitrogenase activity assay, revealed that all isolates contained nitrogen-fixing abilities. In addition, most of them have cellulase activity which is one of the common features of endophytic bacteria from plant.

ISSN 

0257-2389

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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