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Title 

Dykellic acid inhibits drug-induced caspase-3-like protease activation

Authors 

Sang-Han LeeEun Soo YoukHo Jae LeeYung Hee KhoHwan Mook KimSung Uk Kim

Publisher 

Elsevier

Issue Date 

2003

Citation 

Biochemical and Biophysical Research Communications, vol. 302, no. 3, pp. 539-544

Keywords 

apoptosis inhibitorcamptothecincaspase-3-like proteasedykellic acidinhibitorcaspase 3f 50733unclassified drugdrug bindingdrug mechanism

Abstract 

Dykellic acid is a novel microbial metabolite isolated from the broth of Westerdykella multispora F50733. Investigations on the molecular function of dykellic acid revealed that this compound partially inhibits calcium influx, resulting in a decrease in Ca2+-dependent endonuclease activation and DNA fragmentation induced by camptothecin. In our experiments, active caspase-3-like protease cleavage of procaspase-3, PARP, and cytosolic cytochrome c was inhibited by dykellic acid in a concentration-dependent manner when the apoptosis was induced by camptothecin as well as doxorubicin. We confirmed that dykellic acid did not bind to camptothecin using surface plasmon resonance analysis. These results suggest that dykellic acid inhibits drug-induced apoptosis via a caspase-3-like protease-suppressing mechanism. Our data provide important information on the mechanism of action of dykellic acid and indicate that this compound may be employed in the treatment of specific caspase-3-like protease-mediated diseases.

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1016/S0006-291X(03)00210-9

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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