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Title 

Production of recombinant hirudin in galactokinase-deficient Saccharomyces cerevisiae by fed-batch fermentation with continuous glucose feeding

Authors 

Srinivas RamisettiHyun Ah KangSang Ki RheeChul Ho Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2003

Citation 

Biotechnology and Bioprocess Engineering, vol. 8, no. 3, pp. 183-186

Keywords 

fed-batch fermentationGAL promotergalactoserecombinant hirudinsaccharomyces cerevisiae

Abstract 

The artificial gene coding for anticoagulant hirudin was placed under the control of the GAL 10 promoter and expressed in the galactokinase-deficient strain (Δgal1) of Saccharomyces cerevisiae, which uses galactose only as a gratuitous inducer in order to avoid its consumption. For efficient production of recombinant hirudin, a carbon source other than galactose should be provided in the medium to support growth of the Δgal1 strain. Here we demonstrate the successful use of glucose in the fed-batch fermentation of the Δgal1 strain to achieve efficient production of recombinant hirudin, with a yield of up to 400 mg hirudin/L.

ISSN 

1226-8372

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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