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Title 

Enhanced stress-tolerance of transgenic tobacco plants expressing a human dehydroascorbate reductase gene

Authors 

Suk Yoon KwonSun Mee ChoiYoung Ock AhnHeang Soon LeeH B LeeY M ParkSang Soo Kwak

Publisher 

Elsevier

Issue Date 

2003

Citation 

Journal of Plant Physiology, vol. 160, no. 4, pp. 347-353

Keywords 

ascorbic aciddehydroascorbate reductaseglutathionemethyl viologenoxidative stresstransgenic tobacco plantgenesoxidationtobaccoplants (botany)

Abstract 

To analyze the physiological role of dehydroascorbate reductase (DHAR, EC 1.8.5.1) catalyzing the reduction of DHA to ascorbate in environmental stress adaptation, T1 transgenic tobacco (Nicotiana tabacum cv. Xanthi) plants expressing a human DHAR gene in chloroplasts were biochemically characterized and tested for responses to various stresses. Fully expanded leaves of transgenic plants had about 2.29 times higher DHAR activity (units/g fresh wt) than non-transgenic (NT) plants. Interestingly, transgenic plants also showed a 1.43 times higher glutathione reductase activity than NT plants. As a result, the ratio of AsA/DHA was changed from 0.21 to 0.48, even though total ascorbate content was not significantly changed. When tobacco leaf discs were subjected to methyl viologen (MV) at 5 μmol/L and hydrogen peroxide (H2O2) at 200 mmol/L, transgenic plants showed about a 40% and 25% reduction in membrane damage relative to NT plants, respectively. Furthermore, transgenic seedlings showed enhanced tolerance to low temperature (15°C) and NaCl (100 mmol/L) compared to NT plants. These results suggest that a human derived DHAR properly works for the protection against oxidative stress in plants.

ISSN 

0176-1617

Link 

http://dx.doi.org/10.1078/0176-1617-00926

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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