상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Involvement of NF-κB in the regulation of S100A6 gene expression in human hepatoblastoma cell line HepG2

Authors 

Joung Hyuck JooJae Wha KimYong Hee LeeSun Young YoonJ H KimS G PaikIn Seong Choe

Publisher 

Elsevier

Issue Date 

2003

Citation 

Biochemical and Biophysical Research Communications, vol. 307, no. 2, pp. 274-280

Keywords 

HepG2NF-κBpromoterS100A6TNFαimmunoglobulin enhancer binding proteincell cycle proteinS100A6 protein, humanbinding sitecancer cell culture

Abstract 

S100A6 (calcyclin) is an acidic calcium binding protein with two EF-hand motifs and overexpressed in several tumors including intrahepatic carcinoma. TNFα, a strong NF-κB activator required for hepatocyte proliferation during liver regeneration, triggered the expression of S100A6 mRNA in human hepatoblastoma cell line HepG2. Transient expression of NF-κB (p65) increased S100A6 promoter activity and expression of inhibitor of NF-κB (IκBα) decreased TNFα-induced S100A6 promoter activity. To confirm the involvement of NF-κB in S100A6 promoter activation, we analyzed serially deleted promoter constructs of the S100A6 gene by luciferase reporter assay and found a NF-κB-responsive DNA fragment at the position between -584 and -361. Electrophoretic mobility shift assays showed that TNFα induced p65 binding to a potential NF-κB binding site at -460/-451. Furthermore, treatment of cells with CAPE (caffeic acid phenethyl ester), a specific NF-κB (p65) inhibitor, decreased NF-κB binding and promoter activity. These results suggest that NF-κB transcription factor contributes to the activation of S100A6 gene expression in response to TNFα in HepG2 cells.

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1016/S0006-291X(03)01199-9

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)