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Title 

Synergistic activation of p70S6 kinase associated with stem cell factor in MO7e cells

Authors 

Yong Hee LeeH E BroxmeyerC MantelH J KwonJae Wha KimJin Sook KimDur Han KwonIn Seong Choe

Publisher 

Korean Society of Medical Biochemistry

Issue Date 

2003

Citation 

Experimental and Molecular Medicine, vol. 35, no. 3, pp. 222-226

Keywords 

hematopoietic progenitorsp70S6KSCF synergytranslational controlgranulocyte macrophage colony stimulating factorS6 kinasestem cell factorcell lineenzyme activationhuman cell

Abstract 

Stem cell factor (SCF) is an early-acting cytokine inducing proliferative synergy with other cytokines in hematopoietic cells. We earlier showed that p21 was synergistically induced in SCF synergy and the p44/42 MAPK pathway was essential for the transcriptional control of p21. SCF synergy accompanies protein synthesis. p70S6K implicated in translational control in many other systems has not been shown in SCF synergy induced system. GM-CSF dependent human cell line MO7e was stimulated with GM-CSF with SCF, and investigated activation of p70S6K by using phospho-specific antibody. A possible contribution of p70S6K to SCF synergy was examined by measuring p21 induction as a model system. p70S6K was slightly activated by GM-CSF alone and markedly activated by SCF alone. Combined stimulation with these two cytokines synergistically activated p70S6K resulting in persistent activation. Addition of the pathway-specific inhibitors for PI3K or FRAP/TOR, two upstream pathways of p70S6K resulted in abolishment of p70S6K phosphorylation and also significant reduction of p21 protein level. These data suggest that synergistically activated p70S6K by GM-CSF plus SCF involves, at least in part, protein translational control including regulation of p21 protein.

ISSN 

1226-3613

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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