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Title 

Isolation and characterization of the TRP1 gene from the yeast Yarrowia lipolytica and multiple gene disruption using a TRP blaster

Authors 

S A CheonE J HanHyun Ah KangD M OgrydziakJ Y Kim

Publisher 

Wiley-Blackwell

Issue Date 

2003

Citation 

Yeast, vol. 20, no. 8, pp. 677-685

Keywords 

5-FAAcounterselectionmultiple gene disruptionTRPIyarrowia lipolyticaanthranilate phosphoribosyltransferaseanthranilic acidanthranilic acid derivativeisomerasefungal DNA

Abstract 

The TRP1 gene encoding N-(5′-phosphoribosyl)-anthranilate isomerase was isolated from the yeast Yarrowia lipolytica, in which only a few genetic marker genes are available. The Y. lipolytica TRP1 gene (YlTRP1) cloned by complementation of Y. lipolytica trp1 mutation was found to be a functional homologue of Saccharomyces cerevisiae TRP1. Since YlTRP1 could be used for counterselection in medium containing 5-fluoroanthranilic acid (5-FAA), we constructed TRP blasters that contained YlTRP1 flanked by a direct repeat of a sequence and allowed the recycling of the YlTRP1 marker. Using the TRP blasters the sequential disruption of target genes could be carried out within the same strain of Y. lipolytica. The nucleotide sequence of the YlTRP1 gene has been deposited at GenBank under Accession No. AF420590.

ISSN 

0749-503X

Link 

http://dx.doi.org/10.1002/yea.987

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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