상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Functional identification of rub52 gene involved in the biosynthesis of rubradirin

Authors 

J MaharjanK LiouH C LeeC G KimJung Joon LeeJ C YooJ K Sohng

Publisher 

Springer Verlag (Germany)

Issue Date 

2003

Citation 

Biotechnology Letters, vol. 25, no. 11, pp. 909-915

Keywords 

D-rubranitrosedeoxygenationheterologous expressionstreptomyces achromogensrubradirinbiosynthesisgene expression profilinggene expression regulationgeneticsmolecular genetics

Abstract 

An open reading frame, rub52, has been identified as a gene encoding thymidine diphospho-glucose 2,3-dehydratase by sequence analysis of the rubradirin biosynthetic gene cluster of Streptomyces achromogenes var. rubradiris NRRL3061. The gene codes for a protein consisting of 458 amino acids with calculated molecular mass of 50 862 Da. The gene was amplified and heterologously expressed in Escherichia coli as a soluble His-tagged fusion protein. C-2 deoxygenation functionality of thymidine diphospho-4-keto-6-deoxyglucose was assigned to the rub52 gene product from in vitro enzyme assay.

ISSN 

0141-5492

Link 

http://dx.doi.org/10.1023/A:1024075902143

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)