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Title 

Expression and characterization of a novel enantioselective lipase from Acinetobacter species SY-01

Authors 

S J HanJ H BackM Y YoonP K ShinC S CheongMoon Hee SungSeung Pyo HongI Y ChungY S Han

Publisher 

Elsevier

Issue Date 

2003

Citation 

Biochimie, vol. 85, no. 5, pp. 501-510

Keywords 

acinetobacter species SY-01cis-(±)-2-(bromomethyl)-2-(2,4-dichlorophenyl )-1,3-dioxolane-4-methyl acetateenantioselectivelipaselipase-specific chaperoneacetic acid derivativeacid lipaseantifungal agentchaperoneitraconazole

Abstract 

A novel lipase gene, lipase A, of Acinetobacter species SY-01 (A. species SY-01) was cloned, sequenced, and expressed in Bacillus subtilis 168. The deduced amino acid (aa) sequences for the lipase A and its chaperone, lipase-specific chaperone, were found to encode mature proteins of 339 aa (37.2 kDa) and 347 aa (38.1 kDa), respectively. The aa sequence of lipase A and lipase-specific chaperone shared high homology 82 and 67% identity with the lipase A and the lipase B of A. species RAG-1. This new lipase was defined as a group I Proteobacterial lipase family. The expressed lipase A was purified through sequential treatment with Q-Sepharose, Resource Q, and Superdex-S75 columns. The maximal activity was observed at 50 °C for hydrolysis of p-nitrophenyl monoesters and found to be stable at pH 9-11, with optimal activity at pH 10. Lipase A hydrolyzed wide range of fatty acid esters of p-nitrophenyl, but preferentially hydrolyzed short length acyl chains (C2 and C4). Moreover, lipase A from A. species SY-01 catalyzed hydrolysis of the two acetate isomers of cis-(±)-2-(bromomethyl)-2-(2,4-dichlorophenyl)-1,3-dioxolane-4-methyl acetate, an intermediate required for the synthesis of Itraconazole which was an anti-fungal drug, at different rate and yielded cis-(-)-isomer in 81.5% conversion with 91.9% enantiomeric excess.

ISSN 

0300-9084

Link 

http://dx.doi.org/10.1016/S0300-9084(03)00057-9

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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