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Title 

High frequency somatic embryogenesis and plant regeneration in petiole and leaf explant cultures and petiole-derived embryogenic cell suspension cultures of Hylomecon vernalis

Authors 

Suk Weon KimD S InTae Jin KimJang Ryol Liu

Publisher 

Springer Verlag (Germany)

Issue Date 

2003

Citation 

Plant Cell Tissue and Organ Culture, vol. 74, no. 2, pp. 163-167

Keywords 

medicinal plantpapaveraceasomatic embryofrequenciesplant cell cultureplant regenerationhylomecon vernalis

Abstract 

Culture conditions are described for high frequency somatic embryogenesis and plant regeneration in petiole and leaf explant cultures and petiole-derived embryogenic cell suspension cultures of Hylomecon vernalis Max. Petiole explants formed embryogenic calluses at a frequency of 53% when cultured on B5 medium supplemented with 13.6 μM 2,4-dichlorophenoxyacetic acid (2,4-D) alone. Leaf explants formed embryogenic calluses at a frequency of 21% when cultured at a combination of 4.52 μM 2,4-D and 2.22 μM 6-benzyladenine. Cell suspension cultures were established with petiole-derived embryogenic calluses using liquid B5 medium with 4.52 μM 2,4-D. Upon plating onto B5 basal medium, cell suspension cultures produced numerous somatic embryos, which then developed into plantlets. Regenerated plantlets were transplanted to potting soil and grown to maturity in a greenhouse.

ISSN 

0167-6857

Link 

http://dx.doi.org/10.1023/A:1023997627578

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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