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Title 

Characterization of Korean cattle Keratin IV gene

Authors 

D Y KimSeong Lan YuB C SangDae Yeul Yu

Publisher 

The Asian-australasian Association of Animal

Issue Date 

2003

Citation 

Asian-Australasian Journal of Animal Sciences, vol. 16, no. 7, pp. 1055-1059

Keywords 

keratin IV GenekeratinocyteKorean CattlePDGF

Abstract 

Keratins, the constituents of epithelial intermediate filaments, are precisely regulated in a tissue and development specific manner. There are two types of keratin in bovine. The type I is acidic keratin and the type II is neutral/basic keratin. 1.5 kb of 5′ flanking sequence of Korean cattle Keratin IV gene, type II keratin (59 kDa), was cloned and sequenced. A symmetrical motif AApuCCAAA are located in a defined region upstream of the TATA box. Proximal SP1, AP1, E-box and CACC elements as the major determinants of transcription are identified. When it was compared to the bovine sequence from -600 bp to ATG upstream, the homology was 97% in nucleotide sequence. Several A and T sequences, located in the promoter region, are deleted in the Korean cattle. An expression vector consisted of Korean cattle Keratin IV gene promoter/SV40 large T antigen was transfected to HaCaT cell (Epithelial keratinocyte). The transformed HaCaT cells showed active proliferation when treated with PDGF (Platelet-derived growth factor) in 0.3% soft agar compared to control cells. These results indicate that Korean cattle Keratin IVgene promoter can be used as a promoter for transfection into epithelial cell.

ISSN 

1011-2367

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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