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Title 

Dexamethasone reverses TGF-β-mediated inhibition of primary rat preadipocyte differentiation

Authors 

Sun Mi ShinKun Yong KimJae Kwang KimSuk Ran YoonIn Pyo ChoiYoung Yang

Publisher 

Elsevier

Issue Date 

2003

Citation 

FEBS Letters, vol. 543, no. 1, pp. 25-30

Keywords 

CCAAT/enhancer binding protein-αdexamethasoneperoxisome proliferator-activated receptor γrat preadipocytetransforming growth factor-βperoxisome proliferator activated receptor alphacell differentiationinhibition kineticsrat

Abstract 

Dexamethasone and transforming growth factor-β (TGF-β) show contrary effects on differentiation of adipocytes. Dexamethasone stimulates adipocyte differentiation whereas TGF-β inhibits it. In the present study, we investigated whether dexamethasone could reverse the TGF-β-mediated inhibition of preadipocyte differentiation. Primary rat preadipocytes, obtained from Sprague-Dawley rats, were pretreated with dexamethasone in the presence or absence of TGF-β, prior to the induction of differentiation. Co-treatment of dexamethasone and TGF-β before inducing differentiation reversed the TGF-β-mediated inhibition of preadipocyte differentiation. In order to elucidate the mechanism by which dexamethasone reversed the effect of TGF-β on the inhibition of preadipocyte differentiation, the expression of CCAAT/enhancer binding protein-α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ) was examined. Dexamethasone increased C/EBPα and PPARγ expression in the absence of TGF-β and also recovered the TGF-β-mediated suppression of C/EBPα expression in preadipocytes. Its effect was sustained in differentiated adipocytes as well. However, those effects were not observed in 3T3-L1 preadipocytes or differentiated adipocytes. These results indicate that dexamethasone reverses the TGF-β-mediated suppression of adipocyte differentiation by regulating the expression of C/EBPα and PPARγ, which is dependent on the cellular context.

ISSN 

0014-5793

Link 

http://dx.doi.org/10.1016/S0014-5793(03)00371-5

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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