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Title 

Age-related increase of peripheral CD4+ CD8+ double-positive T lymphocytes in cynomolgus monkeys: longitudinal study in relation to thymic involution

Authors 

W W LeeKi Hoan NamK TeraoH AkariY Yoshikawa

Publisher 

Wiley-Blackwell

Issue Date 

2003

Citation 

Immunology, vol. 109, no. 2, pp. 217-225

Keywords 

beta1 integrinCD28 antigenCD4 antigenCD8 antigenT lymphocyte receptoragecell selectioncorrelation analysisenzyme linked immunosorbent assayimmune system

Abstract 

The age-related increase of peripheral CD4+ CD8+ double-positive (DP) T cells in cynomolgus monkeys has been reported previously. Because the percentage of DP T cells in cynomolgus monkeys increases abruptly in parallel with the thymic involution occurring at around 11 years of age, it was suggested that thymic involution was associated with this increase. Therefore, a longitudinal study was carried out over 5 years to clarify the exact time when DP T lymphocytes start to increase in relation to the thymic involution. Twelve cynomolgus monkeys at 6 years of age were classified into three groups, based on their percentage of DP T cells, as follows: DP-High (>5% DP T cells); DP-Middle (1-5% DP T cells); and DP-Low (<1% DP T cells). In the DP-High group, the percentage of DP T cells showed an abrupt increase, of > 10%, in monkeys at 7 years of age, and the prevalence of this subset correlated with a distinctive increase in the percentage of memory T cells (CD4+ CD29high, CD8+ CD28-), indicating an association with the maturation of immune function, including thymic involution. To assess the thymic function, the coding joint of T-cell receptor excision circles (cjTREC) levels in sorted T cells were analysed by polymerase chain reaction (PCR)-enzyme-linked immunosorbent assay (ELISA). The cjTREC in the T cells of the DP-High group (4362 ± 3139 copies/105 T cells) was significantly lower than that (22 722 ± 4928 copies/105 T cells) of the DP-Low group. Moreover, the mean copy number of cjTREC in naive T cells was also significantly different between the DP-High and the DP-Low group (0.457 ± 0.181 and 1.141 ± 0.107, respectively). These findings suggest that thymic involution has an influence on the age-related increase of DP T cells in cynomolgus monkeys.

ISSN 

0019-2805

Link 

http://dx.doi.org/10.1046/j.1365-2567.2003.01646.x

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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