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Title 

Identification of genes for biosynthesis of antibacterial compound from Pseudomonas fluorescens B16, and its activity against Ralstonia solanacearum

Authors 

J W KimJ G KimByoung Keun ParkO H ChoiC S ParkI G Hwang

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2003

Citation 

Journal of Microbiology and Biotechnology, vol. 13, no. 2, pp. 292-300

Keywords 

antibacterial activitybiological controlP. flurorescensantibiotic biosynthesisgene deletiongene identificationgene librarygene locationgenetic complementationmutagenesis

Abstract 

Pseudomonas fluorescens B16 is a plant growth-promoting rhizobacterium, which produces an antibacterial compound that is effective against plant root pathogens, such as Agrobacterium tumefaciens and Ralstonia solanacearum. We mutagenized the strain B16 with Omegon-Km and isolated six antibacterial-activity-deficient mutants. Two cosmid clones that hybridized with the mutant clones also were isolated from a genomic library of the parent strain. Using deletion and complementation analyses, it was found that the biosynthesis genes resided in a 4.3-kb SalI-NarI fragment. When a plasmid clone carrying the fragment was introduced into P. fluorescens strain 1855.344, which does not exhibit any antibacterial activity, the transconjugants exhibited antibacterial activity, indicating that the plasmid clone carried all the genes essential for production of the antibacterial compound. DNA sequence analysis of the fragment identified four putative open reading frames (ORFs): orf1 through orf4. The deduced amino acid sequences of ORF1, ORF2, and ORF4 were similar to cystathionine gamma lyase, pyruvate formate-lyase activating enzyme, and transcriptional regulator, respectively, yet the amino acid sequence of ORF3 showed no similarities to any known proteins. It was also demonstrated that the antibacterial activity was responsible for biological control of the bacterial wilt caused by R. solanacearum.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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