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Title 

Drug-induced haploinsufficiency of fission yeast provides a powerful tool for identification of drug targets

Authors 

Jo Young ParkYoung Joo JangS J YouYoung Sook KilEun Jung KangJee Hee AhnYoung Kwon RyooMin Youn LeeS Y ParkH J LeeJ Y KimS H KimW S YangK B SongH M ParkY J ChungH B KimKwang Lae HoeKyung Sook ChungDong Uk KimHyang Sook YooMi Sun Won

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2003

Citation 

Journal of Microbiology and Biotechnology, vol. 13, no. 2, pp. 317-320

Keywords 

drug targethaploinsufficiencyPCR-based targeted mutagenesisschizosaccharomyces pombetiabendazoledrug researchdrug sensitivitydrug targetingfungus mutantgene mutation

Abstract 

Genome-wide systematic deletion mutants were generated using a PCR-based targeted mutagenesis of Schizosaccharomyces pombe. In a drug-sensitivity assay using thiabendazole (TBZ), an inhibitor of microtubule assembly, a heterozygous nda2 mutant (nda2+/nda2-), deleting one copy of nda2 encoding the microtubule subunit alpha 1 demonstrated a distinct sensitivity to TBZ, indicating TBZ-induced haploinsufficiency. This result suggests that profiling drug-induced haploinsufficiency can be exploited to identify target genes for drugs and discover new drugs.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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